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STING in Ras V12 , scrib − / − clones plays a key role in controlling the production of large EVs . (a) Images of eye discs with Ras V12 (a’) or Ras V12 , scrib −/− clones (a“‐a”″). Arrowheads indicate the features associated with CIN: a“: chromatin bridge, a”’: micronucleus, and a“”: cytosolic leakage of chromatin. Clonal cells are marked with GFP (green), the nuclear envelope is stained <t>with</t> <t>anti‐Lamin</t> antibody (magenta), and DNA is stained with DAPI (grey). (b) Relative mRNA expression of STING target genes in eye‐antennal discs. Ras V12 discs were prepared at 6 d AEL. Other discs were prepared at 8 d AEL. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (c) Still shots from ex vivo live imaging of eye discs. Arrowheads indicate blebs and EVs. See for full movies. (d) Quantification of EVs generated from eye discs from 8 d AEL larvae. EVs were quantified at 2 h post incubation. N = 3 for each genotype. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (e) Schematic of experiments used to assess the expression of antimicrobial peptides in the fat body upon manipulation of Sting in eye disc Ras V12 , scrib −/− clones. (f) Relative mRNA expression of antimicrobial peptides in the fat body. Fat body from larvae with Ras V12 eye disc clones was prepared from 6 d AEL larvae. Fat body from other genotypes was prepared from 8 d AEL larvae. Mean ± SEMs are shown. **p < 0.01, *** p < 0.001 one‐way ANOVA.
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STING in Ras V12 , scrib − / − clones plays a key role in controlling the production of large EVs . (a) Images of eye discs with Ras V12 (a’) or Ras V12 , scrib −/− clones (a“‐a”″). Arrowheads indicate the features associated with CIN: a“: chromatin bridge, a”’: micronucleus, and a“”: cytosolic leakage of chromatin. Clonal cells are marked with GFP (green), the nuclear envelope is stained <t>with</t> <t>anti‐Lamin</t> antibody (magenta), and DNA is stained with DAPI (grey). (b) Relative mRNA expression of STING target genes in eye‐antennal discs. Ras V12 discs were prepared at 6 d AEL. Other discs were prepared at 8 d AEL. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (c) Still shots from ex vivo live imaging of eye discs. Arrowheads indicate blebs and EVs. See for full movies. (d) Quantification of EVs generated from eye discs from 8 d AEL larvae. EVs were quantified at 2 h post incubation. N = 3 for each genotype. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (e) Schematic of experiments used to assess the expression of antimicrobial peptides in the fat body upon manipulation of Sting in eye disc Ras V12 , scrib −/− clones. (f) Relative mRNA expression of antimicrobial peptides in the fat body. Fat body from larvae with Ras V12 eye disc clones was prepared from 6 d AEL larvae. Fat body from other genotypes was prepared from 8 d AEL larvae. Mean ± SEMs are shown. **p < 0.01, *** p < 0.001 one‐way ANOVA.
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STING in Ras V12 , scrib − / − clones plays a key role in controlling the production of large EVs . (a) Images of eye discs with Ras V12 (a’) or Ras V12 , scrib −/− clones (a“‐a”″). Arrowheads indicate the features associated with CIN: a“: chromatin bridge, a”’: micronucleus, and a“”: cytosolic leakage of chromatin. Clonal cells are marked with GFP (green), the nuclear envelope is stained <t>with</t> <t>anti‐Lamin</t> antibody (magenta), and DNA is stained with DAPI (grey). (b) Relative mRNA expression of STING target genes in eye‐antennal discs. Ras V12 discs were prepared at 6 d AEL. Other discs were prepared at 8 d AEL. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (c) Still shots from ex vivo live imaging of eye discs. Arrowheads indicate blebs and EVs. See for full movies. (d) Quantification of EVs generated from eye discs from 8 d AEL larvae. EVs were quantified at 2 h post incubation. N = 3 for each genotype. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (e) Schematic of experiments used to assess the expression of antimicrobial peptides in the fat body upon manipulation of Sting in eye disc Ras V12 , scrib −/− clones. (f) Relative mRNA expression of antimicrobial peptides in the fat body. Fat body from larvae with Ras V12 eye disc clones was prepared from 6 d AEL larvae. Fat body from other genotypes was prepared from 8 d AEL larvae. Mean ± SEMs are shown. **p < 0.01, *** p < 0.001 one‐way ANOVA.
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STING in Ras V12 , scrib − / − clones plays a key role in controlling the production of large EVs . (a) Images of eye discs with Ras V12 (a’) or Ras V12 , scrib −/− clones (a“‐a”″). Arrowheads indicate the features associated with CIN: a“: chromatin bridge, a”’: micronucleus, and a“”: cytosolic leakage of chromatin. Clonal cells are marked with GFP (green), the nuclear envelope is stained <t>with</t> <t>anti‐Lamin</t> antibody (magenta), and DNA is stained with DAPI (grey). (b) Relative mRNA expression of STING target genes in eye‐antennal discs. Ras V12 discs were prepared at 6 d AEL. Other discs were prepared at 8 d AEL. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (c) Still shots from ex vivo live imaging of eye discs. Arrowheads indicate blebs and EVs. See for full movies. (d) Quantification of EVs generated from eye discs from 8 d AEL larvae. EVs were quantified at 2 h post incubation. N = 3 for each genotype. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (e) Schematic of experiments used to assess the expression of antimicrobial peptides in the fat body upon manipulation of Sting in eye disc Ras V12 , scrib −/− clones. (f) Relative mRNA expression of antimicrobial peptides in the fat body. Fat body from larvae with Ras V12 eye disc clones was prepared from 6 d AEL larvae. Fat body from other genotypes was prepared from 8 d AEL larvae. Mean ± SEMs are shown. **p < 0.01, *** p < 0.001 one‐way ANOVA.
Mouse Anti Lamin B1 B 10, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STING in Ras V12 , scrib − / − clones plays a key role in controlling the production of large EVs . (a) Images of eye discs with Ras V12 (a’) or Ras V12 , scrib −/− clones (a“‐a”″). Arrowheads indicate the features associated with CIN: a“: chromatin bridge, a”’: micronucleus, and a“”: cytosolic leakage of chromatin. Clonal cells are marked with GFP (green), the nuclear envelope is stained <t>with</t> <t>anti‐Lamin</t> antibody (magenta), and DNA is stained with DAPI (grey). (b) Relative mRNA expression of STING target genes in eye‐antennal discs. Ras V12 discs were prepared at 6 d AEL. Other discs were prepared at 8 d AEL. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (c) Still shots from ex vivo live imaging of eye discs. Arrowheads indicate blebs and EVs. See for full movies. (d) Quantification of EVs generated from eye discs from 8 d AEL larvae. EVs were quantified at 2 h post incubation. N = 3 for each genotype. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (e) Schematic of experiments used to assess the expression of antimicrobial peptides in the fat body upon manipulation of Sting in eye disc Ras V12 , scrib −/− clones. (f) Relative mRNA expression of antimicrobial peptides in the fat body. Fat body from larvae with Ras V12 eye disc clones was prepared from 6 d AEL larvae. Fat body from other genotypes was prepared from 8 d AEL larvae. Mean ± SEMs are shown. **p < 0.01, *** p < 0.001 one‐way ANOVA.
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STING in Ras V12 , scrib − / − clones plays a key role in controlling the production of large EVs . (a) Images of eye discs with Ras V12 (a’) or Ras V12 , scrib −/− clones (a“‐a”″). Arrowheads indicate the features associated with CIN: a“: chromatin bridge, a”’: micronucleus, and a“”: cytosolic leakage of chromatin. Clonal cells are marked with GFP (green), the nuclear envelope is stained <t>with</t> <t>anti‐Lamin</t> antibody (magenta), and DNA is stained with DAPI (grey). (b) Relative mRNA expression of STING target genes in eye‐antennal discs. Ras V12 discs were prepared at 6 d AEL. Other discs were prepared at 8 d AEL. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (c) Still shots from ex vivo live imaging of eye discs. Arrowheads indicate blebs and EVs. See for full movies. (d) Quantification of EVs generated from eye discs from 8 d AEL larvae. EVs were quantified at 2 h post incubation. N = 3 for each genotype. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (e) Schematic of experiments used to assess the expression of antimicrobial peptides in the fat body upon manipulation of Sting in eye disc Ras V12 , scrib −/− clones. (f) Relative mRNA expression of antimicrobial peptides in the fat body. Fat body from larvae with Ras V12 eye disc clones was prepared from 6 d AEL larvae. Fat body from other genotypes was prepared from 8 d AEL larvae. Mean ± SEMs are shown. **p < 0.01, *** p < 0.001 one‐way ANOVA.
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Developmental Studies Hybridoma Bank lamin c
STING in Ras V12 , scrib − / − clones plays a key role in controlling the production of large EVs . (a) Images of eye discs with Ras V12 (a’) or Ras V12 , scrib −/− clones (a“‐a”″). Arrowheads indicate the features associated with CIN: a“: chromatin bridge, a”’: micronucleus, and a“”: cytosolic leakage of chromatin. Clonal cells are marked with GFP (green), the nuclear envelope is stained <t>with</t> <t>anti‐Lamin</t> antibody (magenta), and DNA is stained with DAPI (grey). (b) Relative mRNA expression of STING target genes in eye‐antennal discs. Ras V12 discs were prepared at 6 d AEL. Other discs were prepared at 8 d AEL. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (c) Still shots from ex vivo live imaging of eye discs. Arrowheads indicate blebs and EVs. See for full movies. (d) Quantification of EVs generated from eye discs from 8 d AEL larvae. EVs were quantified at 2 h post incubation. N = 3 for each genotype. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (e) Schematic of experiments used to assess the expression of antimicrobial peptides in the fat body upon manipulation of Sting in eye disc Ras V12 , scrib −/− clones. (f) Relative mRNA expression of antimicrobial peptides in the fat body. Fat body from larvae with Ras V12 eye disc clones was prepared from 6 d AEL larvae. Fat body from other genotypes was prepared from 8 d AEL larvae. Mean ± SEMs are shown. **p < 0.01, *** p < 0.001 one‐way ANOVA.
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Developmental Studies Hybridoma Bank mouse anti lamin
STING in Ras V12 , scrib − / − clones plays a key role in controlling the production of large EVs . (a) Images of eye discs with Ras V12 (a’) or Ras V12 , scrib −/− clones (a“‐a”″). Arrowheads indicate the features associated with CIN: a“: chromatin bridge, a”’: micronucleus, and a“”: cytosolic leakage of chromatin. Clonal cells are marked with GFP (green), the nuclear envelope is stained <t>with</t> <t>anti‐Lamin</t> antibody (magenta), and DNA is stained with DAPI (grey). (b) Relative mRNA expression of STING target genes in eye‐antennal discs. Ras V12 discs were prepared at 6 d AEL. Other discs were prepared at 8 d AEL. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (c) Still shots from ex vivo live imaging of eye discs. Arrowheads indicate blebs and EVs. See for full movies. (d) Quantification of EVs generated from eye discs from 8 d AEL larvae. EVs were quantified at 2 h post incubation. N = 3 for each genotype. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (e) Schematic of experiments used to assess the expression of antimicrobial peptides in the fat body upon manipulation of Sting in eye disc Ras V12 , scrib −/− clones. (f) Relative mRNA expression of antimicrobial peptides in the fat body. Fat body from larvae with Ras V12 eye disc clones was prepared from 6 d AEL larvae. Fat body from other genotypes was prepared from 8 d AEL larvae. Mean ± SEMs are shown. **p < 0.01, *** p < 0.001 one‐way ANOVA.
Mouse Anti Lamin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STING in Ras V12 , scrib − / − clones plays a key role in controlling the production of large EVs . (a) Images of eye discs with Ras V12 (a’) or Ras V12 , scrib −/− clones (a“‐a”″). Arrowheads indicate the features associated with CIN: a“: chromatin bridge, a”’: micronucleus, and a“”: cytosolic leakage of chromatin. Clonal cells are marked with GFP (green), the nuclear envelope is stained with anti‐Lamin antibody (magenta), and DNA is stained with DAPI (grey). (b) Relative mRNA expression of STING target genes in eye‐antennal discs. Ras V12 discs were prepared at 6 d AEL. Other discs were prepared at 8 d AEL. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (c) Still shots from ex vivo live imaging of eye discs. Arrowheads indicate blebs and EVs. See for full movies. (d) Quantification of EVs generated from eye discs from 8 d AEL larvae. EVs were quantified at 2 h post incubation. N = 3 for each genotype. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (e) Schematic of experiments used to assess the expression of antimicrobial peptides in the fat body upon manipulation of Sting in eye disc Ras V12 , scrib −/− clones. (f) Relative mRNA expression of antimicrobial peptides in the fat body. Fat body from larvae with Ras V12 eye disc clones was prepared from 6 d AEL larvae. Fat body from other genotypes was prepared from 8 d AEL larvae. Mean ± SEMs are shown. **p < 0.01, *** p < 0.001 one‐way ANOVA.

Journal: Journal of Extracellular Vesicles

Article Title: Systemic Propagation of STING Signalling via Generation of Large Extracellular Vesicles

doi: 10.1002/jev2.70289

Figure Lengend Snippet: STING in Ras V12 , scrib − / − clones plays a key role in controlling the production of large EVs . (a) Images of eye discs with Ras V12 (a’) or Ras V12 , scrib −/− clones (a“‐a”″). Arrowheads indicate the features associated with CIN: a“: chromatin bridge, a”’: micronucleus, and a“”: cytosolic leakage of chromatin. Clonal cells are marked with GFP (green), the nuclear envelope is stained with anti‐Lamin antibody (magenta), and DNA is stained with DAPI (grey). (b) Relative mRNA expression of STING target genes in eye‐antennal discs. Ras V12 discs were prepared at 6 d AEL. Other discs were prepared at 8 d AEL. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (c) Still shots from ex vivo live imaging of eye discs. Arrowheads indicate blebs and EVs. See for full movies. (d) Quantification of EVs generated from eye discs from 8 d AEL larvae. EVs were quantified at 2 h post incubation. N = 3 for each genotype. Mean ± SEMs are shown. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA. (e) Schematic of experiments used to assess the expression of antimicrobial peptides in the fat body upon manipulation of Sting in eye disc Ras V12 , scrib −/− clones. (f) Relative mRNA expression of antimicrobial peptides in the fat body. Fat body from larvae with Ras V12 eye disc clones was prepared from 6 d AEL larvae. Fat body from other genotypes was prepared from 8 d AEL larvae. Mean ± SEMs are shown. **p < 0.01, *** p < 0.001 one‐way ANOVA.

Article Snippet: Primary antibodies included anti‐pJNK (1:200, Cell Signalling, 4668), anti‐pFak (1:200, Cell Signalling, 700255), and anti‐lamin (1:1000, DSHB, ADL67.10).

Techniques: Clone Assay, Staining, Expressing, Ex Vivo, Imaging, Generated, Incubation